Brought to you by: Mary Beth M.
The process, or technique, of making body tissue grow in a culture medium outside the organism. Tissue culture is often a generic term that refers to both organ culture and cell culture. The terms are often used interchangeably. This was first developed by Ross Granville Harrison. Cell cultures are derived from either primary tissue explants or cell suspensions. Primary cell cultures typically will have a finite life span in culture. Continuous cell lines are abnormal and are often transformed cell lines.
Cells are harvested when the cells have reached a population density which suppresses growth. Ideally, cells are harvested when they are in a semi-confluent state and are still in log phase.
Storing: Liquid N2 is used to preserve tissue culture cells, either in the liquid phase (-196°C) or in the vapor phase (-156°C). Freezing can be lethal to cells due to the effects of damage by ice crystals, alterations in the concentration of electrolytes, dehydration, and changes in pH.
Many of the tissues that are grown in tissue cultures are then used in transplant surgery. Alexis Carrel (see link below)was the man behind this surgery. He won the Nobel Peace Prize in Physiology, or Medicine. He conducted his research that lead to his Nobel Peace Prize at Rockefeller Institute for Medical Research in New York.
More on the Process
Ross Granville Harrison
Tissue Culture in Modern Society